LOCUS       sequence-136087-        9048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Noma K, Jin Y
  TITLE     Optogenetic mutagenesis in Caenorhabditis elegans.
  JOURNAL   Nat Commun. 2015 Dec 3;6:8868. doi: 10.1038/ncomms9868.
  PUBMED    26632265
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-3"; volume: "6:8868. doi"; pages: " 
            10.1038/ncomms9868"
COMMENT     Sequence Label: pCZ886
FEATURES             Location/Qualifiers
     source          1..9048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3836..3853
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3837..3853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4852..5169
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
     terminator      5170..5493
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             7547..7558
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2341 gtcacgtgtg tttttgaaca cggcgatatg tatctgtaga tcatccccaa ttttcaacaa
     2401 tgaatctaaa tactgaactt tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc
     2461 gattttccga ttatacccaa aatatctcag ctcaatgagc acctataaaa atgtaaaaat
     2521 aaagaaagta gtcgaaccat attttgtaaa ggctaaaaaa ctgaataaat ttttatccat
     2581 ccagcgcggc gttacaatcc gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca
     2641 gtctgtttgc gcgacatcga tctggcaaaa atcctaaaaa cgactatgcg tggctcactt
     2701 tatattaata tgaaaaatac aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag
     2761 aagtggaggc cttgttgtcg atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa
     2821 cgctctcaat cttggatatg atataaattc tggataagat atgatggata tgatctatgt
     2881 ttgatagagt gcatttgaaa ggataacggc cctgcagaca tttttgaaaa aataacaaac
     2941 atttaatttt ctcactcgtt taggctattc ccctattttt gatattcctt cgcacatatg
     3001 aaaactactt tttttcgaaa ctgttaactc cagaatttat aaatctatag cccttacttg
     3061 attatttatt atcatggtta ctcacctcat gtctctttct cttttcaaga agtcttcgtt
     3121 catatctgtt gtctctttcc tccttttgaa tctctgcttt gctcttcgcc attgtttcct
     3181 gaaaataatg taacttgaat tgtgtaatat acttttttaa tttgaatttg gcttgtaacg
     3241 cggaatcact acgtgcggga tcatttctta ctagaaaacc cagaaaatgc catatttcac
     3301 ttatctcggg gtcatttcta attagaaaag ctacaaaaac catttctaat tacggtgact
     3361 ttagaactgg tcgaaatagc taattagaaa tgggtttttg ctaattagag gtgactctat
     3421 gttatctgag taagcctcgt ttgtatgtaa actcacagta ctaataaatg caagacaccc
     3481 gggtttgtct agatatgaaa taattgaaat atcaattctg acagacaata atggtaatct
     3541 tgataaggag ttccacgccc aggagaacac gttagttttc ttgtttttga ttgcgtgcgt
     3601 tattttggag aaaaactcga ttttttacaa aataattttt tgaaaggaac actgttcaat
     3661 aagttttgtc ttttttctca gttgtgatac ggttttttat tcttttttgt agttatacag
     3721 aagaccgtta cgaaacagac tgatatcgaa acaaacactg atcttacttg cacttataat
     3781 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgagtgtaa
     3841 aacgacggcc agtgtacgac tcactatagg gcgaattgaa atatcagttt ttaaaaaatt
     3901 aaaccataaa acaaataata taacccaatt tttacatcaa accacaagaa aaaaatacat
     3961 ttgggcccac ggataaagaa attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa
     4021 ttcatttggg tcttaccgcg tataccgtac tccgtttgtt tgatcatttt tgtcagcgct
     4081 ggcggttgtt ttttcatttc atttctgctt caaagacgtt ttctcgaata atttttcgtt
     4141 tattctcttt tttaaaatta atttctagcc gtaaatgtta taaattcacc catttaacgc
     4201 aaatttcatg gtaatctcat ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc
     4261 aaaaattccc cgactttccc caaaatcctg ctcgattttc cgttttctca ttgtattctc
     4321 tcttaattaa ttttatcgat aatcaattga atgtttcaga cagagaatgg ctcgtaccaa
     4381 gcaaaccgct cgtaaatcca ccggaggaaa ggctccaaga aagcagttgg ccaccaaggc
     4441 cgcccgcaaa tcggctccaa ccaccggagg agtcaagaag ccacatcgtt accgtccagg
     4501 aactgtcgct ctccgtgaga ttcgtcgtta ccagaagtcg actgagcttc tcatccgcaa
     4561 gcttcctttc caacgtctcg ttcgtgagat tgcccaggac ttcaagactg atctccgctt
     4621 ccagtcggct gccatcggag ctctccaggt ttgtagtttg aatttgaaat ttaaaaaaaa
     4681 ttaattaaaa tctttttttt tttccaggag gcatctgaag catacctcgt cggactcttc
     4741 gaggacacca acctgtgcgc catccacgcc aagcgcgtca ccatcatgcc aaaggacatg
     4801 caactcgcca gacgcatccg cggagaacgt gcttcgagac cggtagctac tgagaaaagt
     4861 ttcgtgataa ctgatccacg gctgccagac aatcccatca tcttcgcatc cgatggcttc
     4921 ctggagctga ccgagtattc cagagaggag atcctgggcc gcaatggccg ctttctgcag
     4981 ggaccagaga cagaccaggc cacagtgcag aagattcgcg atgccattag agatcagcgc
     5041 gagattaccg tgcagctgat aaactacaca aaaagcggga agaaattctg gaacctcctg
     5101 cacctccagc ccatgaggga ccagaagggt gagctccagt atttcatcgg agtgcagctg
     5161 gatggataaa tgcaagatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
     5221 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
     5281 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
     5341 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
     5401 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
     5461 aggaaacttt attgtgccgc caagaaaaaa gtctcattgg gtcatagctg ttcctgccta
     5521 ggtgagctct ggtaccctct agtcaaggcc ttaagtgagt cgtattacgt agcccgggcc
     5581 tagttctaga cattctctaa tgaaaaaatc tttcagttga aattgaaaat gagttaaagt
     5641 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga
     5701 cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga
     5761 gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag
     5821 ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat
     5881 catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt
     5941 cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt
     6001 tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat
     6061 agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac
     6121 acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga
     6181 tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag
     6241 caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt
     6301 tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact
     6361 tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt
     6421 tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg
     6481 tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg
     6541 gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga
     6601 aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca
     6661 cctgcgacgg gaaggtcgcc gagccgggtg gaatcgatcg ttgttgctcg gctttcatat
     6721 cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt
     6781 gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa
     6841 aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat
     6901 gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt
     6961 tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctattg
     7021 cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg
     7081 aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc
     7141 gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac
     7201 agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga
     7261 agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca
     7321 gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt
     7381 atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag
     7441 ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca
     7501 tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc
     7561 taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca
     7621 aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa tactttttac
     7681 aaggacttgg ataaattggc tcaaagacga agtgagtttg ctaccatcat ggaatcagga
     7741 ggacatttcg acaatgtcat ataatgtttg aaaaatgatt ttaacaaggg tctcattcga
     7801 gataagcaat gctctgagca agtggctcgt cgtcttcaag aatctcttgt tgatattcat
     7861 ttggatcctg ttttgcacga agcatgtgcc atggatattc agagtttttg caaaaaagtt
     7921 ccgccaggtc aaacaatctt tttaattttt atatgatact tcgggtgtat ttttcttctt
     7981 tttcctgttc attttcgctt ttttctcact tttttgtaac aaaatctctc acttttctgt
     8041 aagtcttaac aaaagttcaa ttggctcgat caagtaaaat ttcggccgta tgaacgtttt
     8101 caaaggtttt catttttttt taagttcatt cacaatatca tgaatctctc gtcctttggt
     8161 ttccggtaag tagcgaaata atataagcat acttataaat gaaggaattg aatatagaat
     8221 aaggaaagca taactaccaa taacggaata tagaggtaat actgtaaatg tactgataac
     8281 caccattaca gtattcattg aatatgccac agattgagca atactacgat gtttttgagg
     8341 taccagttca gaggatataa accacgaaat tggaccaact ccagttctga aatttcatta
     8401 ttcgattaaa tcaaaatata tttgaaaaac ctcacccata agtataaccg tagatgatca
     8461 atgcaactag gcatccgtat ttcatcgggt caatcagaat acgtattttt gcgaagaata
     8521 cgaatgcaat tagagctaaa ttattgagaa atgtgaagag aaggagaagc ttgcgtcgac
     8581 caactctgta aacttattca tacaattttg tcttcaaatt ttgaacgttt aaaactatct
     8641 tattcaatga gttagcgggt ttttatagtg agttagttca cgattatcta ataaaactat
     8701 gtccaaacat cctaactgcg accgtattct cactaactcg tcttaaaaac gttgagatta
     8761 ttttaaaccc ttcttcagta caactacagt actgatgcac aaatacaata ataataatac
     8821 attcagacat actctgaaca atgtaacttg tttttgttat actgtggtaa catttattga
     8881 aatgaaatat aaccttactt ttcaatcaca attccaccag taattgttcc agaaacataa
     8941 gctaaaccca ttgcagttgt actccattca gcaatttctg actcaacatt tgcattttct
     9001 agaaagaaag tagaggataa aagaagagcc cataatccga cagggccc
//
