LOCUS       sequence-136100-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry for monosynaptic circuit mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..720
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(155..173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     423..442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaagccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
       61 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      121 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      181 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      241 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      301 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      361 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      421 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      481 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      541 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      601 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      661 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
//
