LOCUS       sequence-136108-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the CORE cassette with KanMX4 and human p53 gene markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Storici F, Resnick MA
  TITLE     Delitto perfetto targeted mutagenesis in yeast with 
            oligonucleotides.
  JOURNAL   Genet Eng (N Y). 2003;25:189-207.
  PUBMED    15260239
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genet Eng 
            (N Y)."; date: "2003"; volume: "25"; pages: "189-207"
COMMENT     Sequence Label: pCORE-Kp53
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..429
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(497..516)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aaggcgcgcc agatctgttt agcttgcctc
       61 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
      121 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
      181 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
      241 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
      301 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
      361 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
      421 taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg
      481 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
      541 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
      601 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
      661 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
      721 gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
      781 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
      841 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
      901 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
      961 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1021 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1081 gtcggaatcg cagaccgata cca
//
