LOCUS       sequence-136114-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Grusch plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQCXIP-EGFP-F
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(162..164,165..167,168..878)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(162..164,165..167,168..878)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(207..228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(468..487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     815..836
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacggacct
       61 gcaggaattg atccgcggcc gcaccggtag gcctcgtacg cttaattaac ggatccggta
      121 ccgaattcgc ggccgcactc gagatgctac cggtcgccac catggtgagc aagggcgagg
      181 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      241 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      301 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      361 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      421 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      481 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      541 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      601 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      661 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      721 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      781 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      841 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctaagctga
      901 accctcct
//
