LOCUS       sequence-136116-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the CORE cassette with convergent KlURA3 and hyg markers 
            and the I-SceI gene under the inducible GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Storici F, Durham CL, Gordenin DA, Resnick MA
  TITLE     Chromosomal site-specific double-strand breaks are efficiently 
            targeted for repair by oligonucleotides in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 
            20.
  PUBMED    14630945
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2003-12-9"; volume: "100(25)"; pages: 
            "14994-9. Epub 2003 Nov 20"
COMMENT     Sequence Label: pGSHU
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggtaccg ggcccggaga caatcatatg ggagaagcaa
       61 ttggaagata gaaaaaaggt actcggtaca taaatatatg tgattctggg tagaagatcg
      121 gtctgcattg gatggtggta acgcattttt ttacacacat tacttgcctc gagcatcaaa
      181 tggtggttat tcgtggatct atatcacgtg atttgcttaa gaattgtcgt tcatggtgac
      241 acttttagct ttgacatgat taagctcatc tcaattgatg ttatctaaag tcatttcaac
      301 tatctaagat gtggttgtga ttgggccatt ttgtgaaagc cagtacgcca gcgtcaatac
      361 actcccgtca attagttgca ccatgtccac aaaatcatat accagtagag ctgagactca
      421 tgcaagtccg gttgcatcga aacttttacg tttaatggat gaaaagaaga ccaatttgtg
      481 tgcttctctt gacgttcgtt cgactgatga gctattgaaa ctagttgaaa cgttgggtcc
      541 atacatttgc cttttgaaaa cacacgttga tatcttggat gatttcagtt atgagggtac
      601 tgtcgttcca ttgaaagcat tggcagagaa atacaagttc ttgatatttg aggacagaaa
      661 attcgccgat atcggtaaca cagtcaaatt acaatataca tcgggcgttt accgtatcgc
      721 agaatggtct gatatcacca acgcccacgg ggttactggt gctggtattg ttgctggctt
      781 gaaacaaggt gcgcaagagg tcaccaaaga accaagggga ttattgatgc ttgctgaatt
      841 gtcttccaag ggttctctag cacacggtga atatactaag ggtaccgttg atattgcaaa
      901 gagtgataaa gatttcgtta ttgggttcat tgctcagaac gatatgggag gaagagaaga
      961 agggtttgat tggctaatca tgaccccagg tgtaggttta gacgacaaag gcgatgcatt
     1021 gggtcagcag tac
//
