LOCUS       sequence-136123-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_alpha1R
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..25)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14..30
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(38..44,45..62,63..68))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    192..216
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(722..741)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcatat
       61 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      121 tgcccgcttt ccagtcggga aacctgtcgt gccagctggg gtctctcgct ctcctgagac
      181 ggatccgtta tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
      241 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
      301 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
      361 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
      421 tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg
      481 atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa
      541 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
      601 gtatctttaa atactgtaga aaagaggaag gaaataataa gttctgttat gagccatatt
      661 caacgggaaa cgtcttgctc aaggccgcga ttaaattcca acatggatgc tgatttatat
      721 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta ccgattgtat
      781 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
      841 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
      901 aagcatttta tccgtactcc tgatgatgca tggttactc
//
