LOCUS       sequence-136124-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_alpha1R
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    4..60
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..78)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..92)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(286..305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    425..449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggcct gcgccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      241 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      301 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      361 ttgtctgctc ccggcatccg cttacagaca agctgtgggt ctctctccgt catgagacgg
      421 atcctgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
      481 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
      541 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
      601 tcggcttctg acgttcagtg cagccgtcat ctgaaaacga catgtcgcac aagtcctaag
      661 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtc
//
