LOCUS       sequence-136129-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate 
            synthetase (AaPrsA-insV2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK630
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             973..990
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1043..1061)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggctaaaa tcgtcgcctg taacagcaac
       61 ctcccacttg ccgaagccgt tgccgcggag ctgaacctcc ccctctgcaa cgccaccgtg
      121 cgccgctttg cggacatgga ggtgtttgta gaaatccacg aaaatgtacg tggtgaggac
      181 gtgttcgtta tccagagcac ctgcaccccc accaatgata acctgatgga actgctgatt
      241 atgctggatg cgctgcgccg tggttcggcc aagcgtatta cagccgttat gccctatttt
      301 gggtatgccc ggcaggatcg taaatctggc ccgcgcacgc ccattagcgc caaactggtg
      361 gcgaatctgt tggtggaagc gggcgccagc cgcgtgctga cactggacct gcacgccatg
      421 cagattcagg gcttttttga tattccggtg gacaatctgt acgccgcgcc cttgtttgtg
      481 cgcgatatcc gctcccatta cggggaccgc gatttaatga ttgtctcccc cgatgtaggt
      541 ggtgtggtgc gtgcccgcca gctggcccag cgcctgaaca cggatctggc cattattgac
      601 aagcggcgtg aacgcgcagg cgtttccgaa gtgatgaacg tgattggtga tgtgcgcggc
      661 cgccactgcc tgctggtaga tgatattgtg gatagcggtg gttcactgtg caatgcagcc
      721 cgcgccattg ccgagcaggg tgccgcatct gtgggcgcat acgtaacgca tggtgtgctg
      781 acaggacacg ccgtggaacg cattgccgat tcgcctatcg aaatgctgac gctgacagac
      841 agcattcgcg ctacagagca ggttatggcc gcacgcaata tccgccagat cactatttct
      901 ggcctgctgg cgcgcgccat ccatgccgtg gcggatgaaa gctccgtttc ctccctgttt
      961 gactgactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
     1021 gaagctgagt tggctgctgc caccgctgag caataactag cataacccc
//
