LOCUS       sequence-136130-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate 
            synthetase (AaPrsA-insV2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK630
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             992..1014
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt cagtcaaaca
       61 gggaggaaac ggagctttca tccgccacgg catggatggc gcgcgccagc aggccagaaa
      121 tagtgatctg gcggatattg cgtgcggcca taacctgctc tgtagcgcga atgctgtctg
      181 tcagcgtcag catttcgata ggcgaatcgg caatgcgttc cacggcgtgt cctgtcagca
      241 caccatgcgt tacgtatgcg cccacagatg cggcaccctg ctcggcaatg gcgcgggctg
      301 cattgcacag tgaaccaccg ctatccacaa tatcatctac cagcaggcag tggcggccgc
      361 gcacatcacc aatcacgttc atcacttcgg aaacgcctgc gcgttcacgc cgcttgtcaa
      421 taatggccag atccgtgttc aggcgctggg ccagctggcg ggcacgcacc acaccaccta
      481 catcggggga gacaatcatt aaatcgcggt ccccgtaatg ggagcggata tcgcgcacaa
      541 acaagggcgc ggcgtacaga ttgtccaccg gaatatcaaa aaagccctga atctgcatgg
      601 cgtgcaggtc cagtgtcagc acgcggctgg cgcccgcttc caccaacaga ttcgccacca
      661 gtttggcgct aatgggcgtg cgcgggccag atttacgatc ctgccgggca tacccaaaat
      721 agggcataac ggctgtaata cgcttggccg aaccacggcg cagcgcatcc agcataatca
      781 gcagttccat caggttatca ttggtggggg tgcaggtgct ctggataacg aacacgtcct
      841 caccacgtac attttcgtgg atttctacaa acacctccat gtccgcaaag cggcgcacgg
      901 tggcgttgca gagggggagg ttcagctccg cggcaacggc ttcggcaagt gggaggttgc
      961 tgttacaggc gacgatttta gccatggtat atctccttct taaagttaaa caaaattatt
     1021 tctagaggga aaccgttgtg gtctccctat a
//
