LOCUS       sequence-136135-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag (AarCH6), additional unique internal 
            restriction enzyme sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK579
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgatg atgggagcat
       61 ggtgcccgta tcaataaacc gctgatgcca agacagagct tccgtcagca gatgcggtgt
      121 gtgcttacca aaggaatttt taagcgcacg atcaaaataa tcctgcaaca tcggccggta
      181 atccggatgc gcacatttgg aaataatttc acgcgcacgt tgcaccggtg aaagcccacg
      241 cagatccgcc agcccctgct cggttacaaa aatctgcgca tcctgcatga tgtggtccac
      301 atgcgcagcc atcggcacaa tggctgaaat cttgccacct ttggctgtgg agggagacag
      361 gaagatggac aggtaggaac tccgggcaaa atcacccgat cccccaatac cattcatcat
      421 tttggaaccc atcacgcggg tagaattcac gtttccgtaa atatccgcct caatcatgcc
      481 gttcatggca atgcagccta aacggcggat aatgccgggg ctgttgctga catcctgctg
      541 gcgcagaatg atcttgctgc ggaagaaatc catccggtta ttgatttctt ccgcagcttc
      601 cgggctcagc gagaaagacg aggcagaggc aatacgcata cggccagaat ccagcatggc
      661 cagcatgcca tcctgaatca cctcactata gcccaccaga ttttcaaatg ggccttcctt
      721 cagcccttcc agcacggcgt tggccacatt gcccacacca gactggagcg gcagaagtga
      781 gggcggcaat cgattctgtt tgacttcatg cccaaagaaa tcaagcaaat agcccgcaat
      841 agcttttgct gtttcatccg gcgcggcaaa cggggcattt ctgtcccggt cattcgtgcg
      901 tacaatggca gcaatcttgt ccgggttcac ccgtaaaacc gggccaccca cgcgctgatc
      961 tgcccgtaca atcggcacaa tatcacgcgt aggcacgcca cttacgttgc catcccagat
     1021 atcatggatg ccttccagca tcggattctg ccattcattt acctcgataa tgaccttttc
     1081 ggccagattg aggaatgtct gggaat
//
