LOCUS       sequence-136137-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX);
            N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR 
            synthetase (AaPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK541
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             833..841
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgctg cgagatcgcg atttactgct
       61 tgcccaactg catgaactcc ggagtgaaca cagagatctg gatacggtta tcaaccgcat
      121 ggcgcatgaa actactttta ttgaccaact ctatctccag cggctgaaaa aacgtaaact
      181 tctgctaaaa gatcagatta ccaaggttga aagccttctg atccctgatg acattgcctg
      241 aacctctccg ttacagactt taaaaaaccg gatagatacc caccgtgagc gaaaccgccc
      301 ccctgccctc tgcttccagt gctttagaag ataaagcagc ctctgccccc gttgtgggca
      361 ttatcatggg cagccagtca gactgggaaa ccatgcgcca tgcagacgca ttgctgacag
      421 agctggaaat tccgcatgaa accctcattg tttcagcaca tcgtacgcca gaccggttgg
      481 cggattatgc gcgtacggca gccgaacgcg gcctgaatgt gattattgct ggggccggtg
      541 gagctgcaca tctgccgggt atgtgtgcgg catggacacg cctgcccgtg ctgggtgttc
      601 cagtagaatc tcgcgctctt aaaggcatgg acagcctgct ttccattgtg cagatgcccg
      661 gcggcgtgcc tgtgggcact ttggctattg gggcatccgg cgccaaaaat gccgccttgc
      721 tggccgcctc cattcttgca ctgtataacc cggcccttgc cgcacgcctt gaaacatggc
      781 gcgcattgca gacggcctct gtccccaact ctcccattac cgaagataaa tgacctcctt
      841 acattctccg cttaagccca atgccgttat tggcattgtg gggggtggcc agcttggccg
      901 catgtctgct gttgctgccg cgcgccttgg ttttcgcacg catattctta cgccagaggc
      961 caacggcccg gcggctcagg tctctcacgc cgtaacatgc ggcaagtatg atgatccgac
     1021 agcactggat gcctttgccc
//
