LOCUS       sequence-136142-        2178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai1-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1-Orai1
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(673..692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             808..1248
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     regulatory      1571..1580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct cactcgagaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag
      121 gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac
      181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 gtgcagtcca tactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
      361 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat ccaggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
      481 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa cacgcgtgac caactgactg aagagcagat cgcagaattt
      841 aaagaggctt tctccctatt tgacaaggac ggggatggga cgataacaac caaggagctg
      901 gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc
      961 aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg
     1021 gcaagaaaaa tgaaagacac agacagtgaa gaagaaatta gagaagcgtt ccgtgtgttt
     1081 gataaggacg gcaatggcta catcggcgca gcagagcttc gccacgtgat gacaaacctt
     1141 ggagagaagt taacagatga agaggttgat gaaatgatca gggtagcaga catcgatggg
     1201 gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagca gatctcgagc
     1261 tcaagcttcg aattccatcc ggagcccgcc ccgcccccga gccgcagcag tcccgagctt
     1321 cccccaagcg gcggcagcac caccagcggc agccgccgga gccgccgccg cagcggggac
     1381 ggggagcccc cgggggcccc gccaccgccg ccgtccgccg tcacctaccc ggactggatc
     1441 ggccagagtt actccgaggt gatgagcctc aacgagcact ccatgcaggc gctgtcctgg
     1501 cgcaagctct acttgagccg cgccaagctt aaagcctcca gccggacctc ggctctgctc
     1561 tccggcttcg ccatggtggc aatggtggag gtgcagctgg acgctgacca cgactaccca
     1621 ccggggctgc tcatcgcctt cagtgcctgc accacagtgc tggtggctgt gcacctgttt
     1681 gcgctcatga tcagcacctg catcctgccc aacatcgagg cggtgagcaa cgtgcacaat
     1741 ctcaactcgg tcaaggagtc cccccatgag cgcatgcacc gccacatcga gctggcctgg
     1801 gccttctcca ccgtcatcgg cacgctgctc ttcctagctg aggtggtgct gctctgctgg
     1861 gtcaagttct tgcccctcaa gaagcagcca ggccagccaa ggcccaccag caagcccccc
     1921 gccagtggcg cagcagccaa cgtcagcacc agcggcatca ccccgggcca ggcagctgcc
     1981 atcgcctcga ccaccatcat ggtgcccttc ggcctgatct ttatcgtctt cgccgtccac
     2041 ttctaccgct cactggttag ccataagacc gaccgacagt tccaggagct caacgagctg
     2101 gcggagtttg cccgcttaca ggaccagctg gaccacagag gggaccaccc cctgacgccc
     2161 ggcagccact atgcctag
//
