LOCUS       sequence-136175-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Franciscella tularensis N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal TEV protease-cleavable His6 
            tag (H6tFtPurE1), synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK569
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             650..667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(720..738)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      734..781
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(871..887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(871..887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(884..906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    895..911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(919..925,926..943,944..949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    964..985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attcaagcgg cgtggatctg ggcaccgaaa atctgtactt tcagtctaac atgtcagttc
      121 aggttggcgt cattatgggc agcaaatctg attggagtac catgaaagaa tgctgtgata
      181 ttctggacaa cctgggcatc ggttatgaat gcgaagtggt tagcgcgcat cgtacgccgg
      241 acaaaatgtt cgattacgca gaaacggcga aagaacgcgg tctgaaagtc atcatcgctg
      301 gcgcgggtgg tgctgcacat ttacctggaa tggttgcggc aaaaactact ttaccagtat
      361 taggtgtacc agttaaatca agtacactta atggccaaga tagtttatta tcaatagtac
      421 agatgccagc gggtattcca gtagctactt ttgcaattgg tatggcagga gcaaaaaacg
      481 cggcgctttt tgctgcaagt atcttgcaac atacagatat aaatattgca aaagcactag
      541 ctgagtttag agctgagcaa acacgatttg ttttggaaaa tcctgatcct agggagcact
      601 aacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      661 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      721 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      781 gctgaaagga ggaactatat ccggatgacc caaatcggat cccgggcccg tcgactgcag
      841 aggcctgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      901 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      961 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1021 aaacctgtcg tg
//
