LOCUS       sequence-136177-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant 
            (AaPurE1fwf-S57C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK585
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             623..640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(693..711)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      707..754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(878..897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgatgagcg aaaccgcccc cctgccctct
       61 gcttccagtg ctttagaaga taaagcagcc tctgcccccg ttgtgggcat tatcatgggc
      121 agccagtcag acttcgaaac catgcgccat gcagacgcat tgctgacaga gctggaaatt
      181 ccgcatgaaa ccctcattgt ttgcgcacat cgtacgccag accggttggc ggattatgcg
      241 cgtacggcag ccgaacgcgg cctgaatgtg attattgctg gggccggtgg agctgcacat
      301 ctgccgggta tgtgtgcggc atggacacgc ctgcccgtgc tgggtgttcc agtagaatct
      361 cgcgctctta aaggcatgga cagcctgctt tccattgtgc agatgcccgg cggcgtgcct
      421 gtgggcactt tggctattgg ggcatccggc gccaaaaatg ccgccttgct ggccgcctcc
      481 attcttgcac tgtataaccc ggcccttgcc gcacgccttg aaacatttcg cgcattgcag
      541 acggcctctg tccccaactc tcccattacc gaagataaat gagaattcga gctccgtcga
      601 caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      661 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      721 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      781 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      961 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1021 cgtagtgggc catcgccctg atagacggtt tt
//
