LOCUS       sequence-136186-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate 
            CoA-transferase, N-terminal His6 tag (H6AaUctD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK551
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgacaaaa gttaaaacgg
      121 ctgcacaggc tgtaagtctt ataaaaaatg gcgccaccat tgcggtaaat tcatcctctg
      181 gcctgctgtg cccagatgcc gtgctggaag ctttgggcca gcgctttgca gcagaaggtg
      241 cccccggcca gcttacaaca attcacccca tctgcgcggg agatatgttt ggcaccaaag
      301 gggtggacca tattgcctat ccgggcatga tccaccgcat tattgggggg tcttacccct
      361 ctggccccac caatgcggcg ccgccactta tttggcagcg tattctgggc gaggatctgg
      421 cagcatataa tgtgccatct ggcattattt ttgacatgct gcgtgagggc gctgcgtgca
      481 ggcccggtgt gctgacaaaa gtggggattg atacctttgc agacccacgg ctggaaggat
      541 gcgccatgaa cgaaacggcg cgccagcagc ctattgtgaa aacaatgcag tttgaaggcg
      601 aggaatggtt gtatttctcg gccctgaagc ccgatgttgc tattattcgt gcgagtaccg
      661 cagatgaaaa gggtaacctg acctttgagc aggaaggcgc aaccctgggc gctatggaaa
      721 tggcgctggc tgcgcataac aatggtgggt tggttattgc ccaggtgcgc cgtgttgcag
      781 cccagggcac gctaaaaccc cataatgtta aagtgccagg cattttggtg gatgtggtgg
      841 tggaagcgcc agagcagctt caaaccacag ccacatttta tgatcccgcc atttcgggcg
      901 agattttccg cccgttatcc agttttgagc ttccggcatt tgggcctgcc aaagttatgg
      961 cacggcgtgt ggcgcaagat ttgcaaaagg actgggccgt taacataggt tttggcattt
     1021 ctgccaatgt gccacgtatt cttttggaag aagggcagca tggtgccgtt acgtgggtga
     1081 tt
//
