LOCUS       sequence-136187-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal 
            His6 tag (H6EcGrxD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK690
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             496..513
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(566..584)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      580..627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      664..1021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(751..770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     961..982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgagcacc actatcgaaa
      121 aaatccaacg ccagattgct gaaaacccga tcctgctgta catgaaaggt tcaccgaaac
      181 tgccgagctg cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt
      241 ttgcctatgt tgatattctg cagaatccgg acattcgtgc ggaactgccg aaatatgcta
      301 actggccgac cttcccgcaa ctgtgggttg acggcgagct ggtcggcggt tgtgatatcg
      361 tgatcgaaat gtatcagcgt ggcgaactgc agcagctgat caaagaaact gccgctaaat
      421 acaagtctga agagccggac gcggaataag gatccgaatt cgagctccgt cgacaagctt
      481 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      541 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      601 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      661 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      961 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1021 t
//
