LOCUS       sequence-136190-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glycinamide ribonucleotide synthetase 
            (EcPurDH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK621
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgaaagta ttagtgattg gtaacggagg
      121 acgtgagcac gcgctggcct ggaaagcggc gcagtcgccg ctggttgaaa ctgtttttgt
      181 tgctccgggt aatgcaggca ctgcgctgga acccgcgcta cagaacgtcg ctatcggcgt
      241 aaccgatatc ccggcgctgt tggatttcgc acaaaacgaa aagattgatc tgaccatcgt
      301 cggcccggaa gcgccgctgg tgaaaggcgt ggtcgatatc ttccgcgccg ccgggctgaa
      361 aatcttcggc ccaaccgcag gtgcggccca actggaaggc tcaaaagcgt ttaccaaaga
      421 tttcctggcc cgccataaga tccctacggc ggaataccag aacttcaccg aggtagaacc
      481 tgcgctggcg tatctgcgtg agaaaggcgc gccaatcgtc attaaagcgg acggtctggc
      541 tgccgggaaa ggcgttatcg tggcgatgac gctggaagaa gcggaagcgg ctgttcacga
      601 tatgctggcg ggcaacgctt ttggcgacgc gggtcatcgc atcgttatcg aagagttcct
      661 cgatggcgaa gaagcgagct ttatcgtgat ggtggacgga gagcatgtgc tgccgatggc
      721 taccagccag gatcacaaac gcgtaggcga taaagatacc ggaccaaaca ccggcgggat
      781 gggcgcttac tcccccgcgc cggtagtaac cgatgaagtt catcagcgca ccatggaacg
      841 tatcatctgg ccaaccgtga aaggcatggc ggcggaaggc aacacctaca ccggttttct
      901 ctacgcgggc ctgatgatcg acaaacaggg caatccgaag gttatcgaat ttaactgccg
      961 ctttggcgat ccggaaaccc agccgattat gctgcgcatg aagtccgatc tggttgagct
     1021 ctgcctggca gcctgtgaag ggaagctgga
//
