LOCUS       sequence-136193-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola ATCC 35405 PurE with a TEV 
            protease-cleavable N-terminal His6 tag (H6tTdPurE2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK636
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggc acaccatcat caccatcatt
       61 caagcggcgt ggatctgggc accgaaaatc tgtactttca gtctcatatg cgtccgctgg
      121 tgattatcct tatgggcagc agttcagata tgggtcacgc cgagaaaatt gcgtctgaat
      181 tgaaaacttt tggtattgag tatgccattc gcatcggctc cgcgcataaa acagcggagc
      241 atgtagtgtc tatgctgaaa gaatatgaag ctttggaccg tcctaaactg tacatcacga
      301 tcgctgggcg tagtaacgct ttatcaggct ttgttgatgg cttcgtcaaa ggtgcaacca
      361 ttgcctgccc accgccaagc gatagcttcg ccggcgccga catttattct tcgttacgga
      421 tgccgagcgg gattagtccg gcactggttc ttgaaccgaa aaatgcggca ctcctggcgg
      481 ctcgtatttt ttcactgtac gataaagaaa tcgcagattc ggtcaaatcc tatatggaat
      541 ccaatgcgca gaaaattatc gaagacgatt cgaaactgaa acgctgaatt cagcgtttca
      601 gtt
//
