LOCUS       sequence-136201-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: 
            CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2372
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata
       61 gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg
      121 aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc
      181 gatagcccta cagttgccta ttctgnccta gtantggcna aagttgagaa gggaaaatcc
      241 aagaaactga agtcagtcna agaattattg gggatnacga ntatggagcg ctcgtctttt
      301 gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa naaggatctc
      361 ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg
      421 gctagcgccg gagagcttca naaggggaac gaactcgcac taccgtctaa atacgtgaat
      481 ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag
      541 aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg
      601 gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac
      661 aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact
      721 cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa
      781 cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga
      841 ttatatgaaa ctcggataga tttgtcacag cttgggggtg acggatcccc caagaa
//
