LOCUS       sequence-136202-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAZ2A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(590..608)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      604..651
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(826..831,832..848,849..854))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(881..899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actttaagaa ggagatatac catgtgcagc agccatcatc atcatcatca cggcagcggc
       61 agcggctctg accaggaggc aaaaccttca actgaggact tgggggataa gaaggaaggt
      121 gaatatatta aactcaaagt cattggacag gatagcagtg agattcactt caaagtgaaa
      181 atgacaacac atctcaagaa actcaaagaa tcatactgtc aaagacaggg tgttccaatg
      241 aattcactca ggtttctctt tgagggtcag agaattgctg ataatcatac tccaaaagaa
      301 ctgggaatgg aggaagaaga tgtgattgaa gtttatcagg aacaaaccgg tggtcatatg
      361 agcgtgaata aagttacctg tctggtttgt cgcaaaggcg ataatgatga atttctgctg
      421 ctgtgtgatg gttgtgatcg tggttgtcat atctattgtc atcgtccgaa aatggaagca
      481 gttccggaag gtgattggtt ttgtaccgtt tgtctggcac agcaggttta atgactcgag
      541 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      601 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      661 ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
      721 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
      781 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
      841 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
      901 ttataggtta atgtcatgat aataatgg
//
