LOCUS       sequence-136203-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK8
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gagcagaagc aagcgtgaca acaattttta
      121 tagtgtagag attggagatt ctacattcac agtcctgaaa cgatatcaga atttaaaacc
      181 tataggctca ggagctcaag gaatagtatg cgcagcttat gatgccattc ttgaaagaaa
      241 tgttgcaatc aagaagctaa gccgaccatt tcagaatcag actcatgcca agcgggccta
      301 cagagagcta gttcttatga aatgtgttaa tcacaaaaat ataattggcc ttttgaatgt
      361 tttcacacca cagaaatccc tagaagaatt tcaagatgtt tacatagtca tggagctcat
      421 ggatgcaaat ctttgccaag tgattcagat ggagctagat catgaaagaa tgtcctacct
      481 tctctatcag atgctgtgtg gaatcaagca ccttcattct gctggaatta ttcatcggga
      541 cttaaagccc agtaatatag tagtaaaatc tgattgcact ttgaagattc ttgacttcgg
      601 tctggccagg actgcaggaa cgagttttat gatgacgcct tatgtagtga ctcgctacta
      661 cagagcaccc gaggtcatcc ttggcatggg ctacaaggaa aacgtggatt tatggtctgt
      721 ggggtgcatt atgggagaaa tggtttgcca caaaatcctc tttccaggaa gggactatat
      781 tgatcagtgg aataaagtta ttgaacagct tggaacacca tgtcctgaat tcatgaagaa
      841 actgcaacca acagtaagga cttacgttga aaacagacct aaatatgctg gatatagctt
      901 t
//
