LOCUS       sequence-136206-         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SLX4
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             455..472
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(525..543)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      539..585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgggccgcac cttgctctcc ctcgggctgc tggttgctga ctttggcgcc atggtcaata
       61 ncccacacct gantgatgtc cagtttcaga cggacagcgg ggaggtgctt tacgcccaca
      121 agttcgtgct ttatgcccga tgcccgctcc tcatccagta tgtgaacaat gaaggcttct
      181 ccgctatcga ggacggggtt gaaacccagc gtgtcctgct gggtgacgtg agcaccgagg
      241 ccgcccgcac gttcctgcac tatctctaca ctgcggacac tggccttcct cctggcctta
      301 gctctgagct gagctccctg gcccacaggt ttggcgtgag tgagctcgtt cacctgtgcg
      361 aacaggtgcc tattgccact gactcagagt gacagtaaag gtggatacgg atccgaattc
      421 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      481 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      541 agcataaccc cttggggcct ctaaacgggt cttgaggggt ttttt
//
