LOCUS       sequence-136208-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TPH2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttagaag gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg
       61 ggtaccgaga acctgtactt ccaatccatg ctagaggatg tgccctggtt ccctcggaag
      121 atctctgagt tagacaaatg ctctcacaga gttctcatgt atggttctga gcttgatgct
      181 gaccacccag gatttaagga caatgtctat cgacagagaa gaaagtattt tgtggatgtg
      241 gccatgggtt ataaatatgg tcagcccatt cccagggtgg agtatactga agaagaaact
      301 aaaacttggg gtgttgtatt ccgggagctc tccaaactct atcccactca tgcttgccga
      361 gagtatttga aaaacttccc tctgctgact aaatactgtg gctacagaga ggacaatgtg
      421 cctcaactcg aagatgtctc catgtttctg aaagaaaggt ctggcttcac ggtgaggccg
      481 gtggctggat acctgagccc acgagacttt ctggcaggac tggcctacag agtgttccac
      541 tgtacccagt acatccggca tggctcagat cccctctaca ccccggaacc agacacatgc
      601 catgaactct tgggacatgt tccactactt gcggatccta agtttgctca gttttcacaa
      661 gaaataggtc tggcgtctct gggagcatca gatgaagatg ttcagaaact agccacgtgc
      721 tatttcttca caatcgagtt tggcctttgc aagcaagaag ggcaactgcg ggcttatgga
      781 gcaggactcc ttt
//
