LOCUS       sequence-136209-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAGEA3
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..378
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             385..405
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttagaag gagatataca tatgcaccat catcatcatc attcttctgg tatgagcgat
       61 aaaattattc acctgactga cgacagtttt gacacggatg tactcaaagc ggacggggcg
      121 atcctcgtcg atttctgggc agagtggtgc ggtccgtgca aaatgatcgc cccgattctg
      181 gatgaaatcg ctgacgaata tcagggcaaa ctgaccgttg caaaactgaa catcgatcaa
      241 aaccctggca ctgcgccgaa atatggcatc cgtggtatcc cgactctgct gctgttcaaa
      301 aacggtgaag tggcggcaac caaagtgggc gcactgtcta aaggtcagtt gaaagagttc
      361 ctcgacgcta acctggccgg taccgagaac ttgtacttcc aatccatgga gttccaagca
      421 gcactcagta ggaaggtggc cgagttggtt cattttctgc tcctcaagta tcgagccagg
      481 gagccggtca caaaggcaga aatgctgggg agtgtcgtcg gaaattggca gtatttcttt
      541 cctgtgatct tcagcaaagc ttccagttcc ttgcagctgg tctttggcat cgagctgatg
      601 gaagtggacc ccatcggcca cttgtacatc tttgccacct gcctgggcct ctcctacgat
      661 ggcctgctgg gtgacaatca gatcatgccc aaggcaggcc tcctgataat cgtcctggcc
      721 ataatcgcaa gagagggcga ctgtgcccct gaggagaaaa tctgggagga gctgagtgtg
      781 ttagaggtgt ttgaggggag ggaagacagt atcttggggg atcccaagaa gctgctcacc
      841 caacatttcg tgcagg
//
