LOCUS       sequence-136211-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttggtt taactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggcggcgg cggcggcggc
      121 gggcgcgggc ccggagatgg tccgcgggca ggtgttcgac gtggggccgc gctacaccaa
      181 cctctcgtac atcggcgagg gcgcctacgg catggtgtgc tctgcttatg ataatgtcaa
      241 caaagttcga gtagctatca agaaaatcag cccctttgag caccagacct actgccagag
      301 aaccctgagg gagataaaaa tcttactgcg cttcagacat gagaacatca ttggaatcaa
      361 tgacattatt cgagcaccaa ccatcgagca aatgaaagat gtatatatag tacaggacct
      421 catggaaaca gatctttaca agctcttgaa gacacaacac ctcagcaatg accatatctg
      481 ctattttctc taccagatcc tcagagggtt aaaatatatc cattcagcta acgttctgca
      541 ccgtgacctc aagccttcca acctgctgct caacaccacc tgtgatctca agatctgtga
      601 ctttggcctg gcccgtgttg cagatccaga ccatgatcac acagggttcc tgacagaata
      661 tgtggccaca cgttggtaca gggctccaga aattatgttg aattccaagg gctacaccaa
      721 gtccattgat atttggtctg taggctgcat tctggcagaa atgctttcta a
//
