LOCUS       sequence-136212-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHC1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             712..729
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(782..800)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      796..843
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggccagctt gggggcgagg agtggacccg
      121 ccacgggagc tttgtcaata agcccacgcg gggctggctg catcccaacg acaaagtcat
      181 gggacccggg gtttcctact tggttcggta catgggttgt gtggaggtcc tccagtcaat
      241 gcgtgccctg gacttcaaca cccggactca ggtcaccagg gaggccatca gtctggtgtg
      301 tgaggctgtg ccgggtgcta agggggcgac aaggaggaga aagccctgta gccgcccgct
      361 cagctctatc ctggggagga gtaacctgaa atttgctgga atgccaatca ctctcaccgt
      421 ctccaccagc agcctcaacc tcatggccgc agactgcaaa cagatcatcg ccaaccacca
      481 catgcaatct atctcatttg catccggcgg ggatccggac acagccgagt atgtcgccta
      541 tgttgccaaa gaccctgtga atcagagagc ctgccacatt ctggagtgtc ccgaagggct
      601 tgcccaggat gtcatcagca ccattggcca ggccttcgag ttgcgctgac agtaaaggtg
      661 gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      721 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      781 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      841 ttgctgaaag gaggaactat atccgga
//
