LOCUS       sequence-136216-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TP53BP1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             475..492
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(545..563)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      559..606
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(730..749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgtcctct ccaggaaata gctttgtagg gctccgtgtt
      121 gtagccaagt ggtcatccaa tggctacttt tactctggga aaatcacacg agatgtcgga
      181 gctgggaagt ataaattgct ctttgatgat gggtacgaat gtgatgtgtt gggcaaagac
      241 attctgttat gtgaccccat cccgctggac actgaagtga cggccctctc ggaggatgag
      301 tatttcagtg caggagtggt gaaaggacat aggaaggagt ctggggaact gtactacagc
      361 attgaaaaag aaggccaaag aaagtggtat aagcgaatgg ctgtcatcct gtccttggag
      421 caaggaaaca gactgagaga gcagtatggg cttggctaag cggccgcact cgagcaccac
      481 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      541 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      601 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      781 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      841 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      901 tttttcgccc
//
