LOCUS       sequence-136218-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD10
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             487..504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(557..575)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(742..761)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggcacgagc cccagctcca aatacgtgaa
      121 gctgaatgtg ggtggagccc tctactatac aaccatgcag acgctgacca agcaggacac
      181 catgctgaag gccatgttaa gcgggcgcat ggaagtgctc accgacagtg aaggctggat
      241 cctcattgac cgctgtggga agcactttgg tacgatactc aactaccttc gagacggggc
      301 ggtgccttta cccgagagcc gccgggagat cgaggagctg ctagcagaag ccaagtacta
      361 cctagtccaa ggcctggtgg aagagtgcca ggcggcccta caaaacaaag atacttatga
      421 gtgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca
      481 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      541 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      601 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      661 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      721 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      781 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      841 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      901 cctgatagac ggtttttcgc cc
//
