LOCUS       sequence-136220-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SEC14L4
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gtcgtcccgt gtgggtgacc tgtcgccgca
      121 acaacaagaa gccctggccc gcttccgtga aaacctgcaa gacctgctgc cgattctgcc
      181 gaacgcggat gactattttc tgctgcgttg gctgcgtgcc cgcaatttcg atctgcagaa
      241 aagtgaagac atgctgcgtc gtcacatgga atttcgcaaa cagcaggatc tggataacat
      301 tgtcacctgg cagccgccgg aagtgatcca actgtatgat tcaggcggtc tgtgcggcta
      361 tgactacgaa ggttgtccgg tgtacttcaa tattatcggc agcctggacc cgaaaggtct
      421 gctgctgagt gcttccaagc aggacatgat tcgtaaacgc atcaaggtgt gcgaactgct
      481 gctgcatgaa tgtgaactgc agacccaaaa actgggccgt aagattgaaa tggcgctgat
      541 ggtttttgat atggaaggtc tgagtctgaa acacctgtgg aagccggcag tggaagttta
      601 tcagcaattt ttctccatcc tggaagctaa ctacccggaa acgctgaaaa atctgattgt
      661 tatccgcgcg ccgaagctgt ttccggtggc cttcaacctg gttaaatcat tcatgtcgga
      721 agaaacccgt cgcaaaattg tgatcctggg cgataattgg aaacaggaac tgacgaagtt
      781 tattagcccg gaccaactgc cggttgaatt tggcggcacc atgaccgatc cggacggtaa
      841 cccgaaatgc ctgaccaaga tcaattatgg cggtgaagtg ccgaaaagct attacctgtg
      901 tgaacaggtt cgtctgcaat acgaacacac gcgttctg
//
