LOCUS       sequence-136223-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHC1
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             652..669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(722..740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      736..783
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(907..926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggctggctg catcccaacg acaaagtcat
      121 gggacccggg gtttcctact tggttcggta catgggttgt gtggaggtcc tccagtcaat
      181 gcgtgccctg gacttcaaca cccggactca ggtcaccagg gaggccatca gtctggtgtg
      241 tgaggctgtg ccgggtgcta agggggcgac aaggaggaga aagccctgta gccgcccgct
      301 cagctctatc ctggggagga gtaacctgaa atttgctgga atgccaatca ctctcaccgt
      361 ctccaccagc agcctcaacc tcatggccgc agactgcaaa cagatcatcg ccaaccacca
      421 catgcaatct atctcatttg catccggcgg ggatccggac acagccgagt atgtcgccta
      481 tgttgccaaa gaccctgtga atcagagagc ctgccacatt ctggagtgtc ccgaagggct
      541 tgcccaggat gtcatcagca ccattggcca ggccttcgag ttgcgctgac agtaaaggtg
      601 gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      661 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      721 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      781 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      841 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      901 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccc
//
