LOCUS       sequence-136232-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The retroviral GOT1 vector was generated by cloning an sgRNA 
            resistant human GOT1 gene block into the pMXS-ires-blast vector by 
            Gibson Assembly..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: PMXS-GOT1
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgacccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt aagccaccat
       61 ggcacctccg tcagtctttg ccgaggttcc gcaggcccag cctgtcctgg tcttcaagct
      121 cactgccgac ttcagggagg atccggaccc ccgcaaggtc aacctgggag tgggagcata
      181 tcgcacggat gactgtcacc catgggtttt gccagtagtg aagaaagtgg agcagaagat
      241 tgctaatgac aatagcctaa atcacgagta tctgccaatc ctgggcctgg ctgagttccg
      301 gagctgtgct tctcgtcttg cccttgggga tgacagccca gcactcaagg agaagcgggt
      361 aggaggtgtg caatctttgg ggggaacagg tgcacttcga attggagctg atttcttagc
      421 gcgttggtac aatggaacaa acaacaagaa cacacctgtc tatgtgtcct caccaacctg
      481 ggagaatcac aatgctgtgt tttccgctgc tggttttaaa gacattcggt cctatcgcta
      541 ctgggatgca gagaagagag gattggacct ccagggcttc ctgaatgatc tggagaatgc
      601 tcctgagttc tccattgttg tcctccacgc ctgtgcacac aacccaactg ggattgaccc
      661 aactccggag cagtggaagc agattgcttc tgtcatgaag caccggtttc tgtttccatt
      721 ttttgattca gcctatcagg gcttcgcatc tggaaacctg gagagagatg cctgggccat
      781 tcgctatttt gtgtctgaag gcttcgagtt cttctgtgcc cagtccttct ccaagaactt
      841 cgggctctac aatgagagag tcgggaatct gactgtggtt ggaaaagaac ctgagagcat
      901 cctgcaagtc ctttcccaga tggagaagat cgtgcggatt acttggtcca atccccccgc
      961 ccagggagc
//
