LOCUS       sequence-136234-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP9
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             719..736
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(789..807)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gaatgcaatg gtcgtgaaca
      121 acctgaccct ccagattgtc cagggccaca ttgaatggca gacggcagat gtaattgtta
      181 attctgtaaa cccacatgat attacagttg gacctgtggc aaagtcaatt ctacaacaag
      241 caggagttga aatgaaatcg gaatttcttg ccacaaaggc taaacagttt caacggtccc
      301 agttggtact ggtcacaaaa ggatttaact tgttctgtaa atatatatac catgtactgt
      361 ggcattcaga atttcctaaa cctcagatat taaaacatgc aatgaaggag tgtttggaaa
      421 aatgcattga gcaaaatata acttccattt cctttcctgc ccttgggact ggaaacatgg
      481 aaataaagaa ggaaacagca gcagagattt tgtttgatga agttttaaca tttgccaaag
      541 accatgtaaa acaccagtta actgtaaaat ttgtgatctt tccaacagat ttggagatat
      601 ataaggcttt cagttctgaa atggcaaaga ggtccaagat gctgagtttg aactgacagt
      661 aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      721 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      781 tgctgccacc gctgagcaat aactagcata accccttggg g
//
