LOCUS       sequence-136250-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of firefly luciferase for in vivo imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Grusch plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQCXIP-F-luc
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(249..267)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac ggacctgcag
       61 gaattgatcc gcggccgcac cggtaggcct cgtacgctta attaacggat ccggggttta
      121 aaccccctcg agatctgcga tctaagtaag cttggcattc cggtactgtt ggtaaagcca
      181 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      241 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      301 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      361 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      421 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      481 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      541 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      601 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      661 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      721 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      781 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      841 cgcatgccag agatcctatt tttggca
//
