LOCUS       sequence-136251-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            Adenovirus production).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pAD/CMV/V5-DEST-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        200..218
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    219..243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        259..366
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             387..404
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             414..443
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttt
      121 aaaggaacca attcagtcga ctggatccgg ttattgtgct gtctcatcat tttggcaaag
      181 aattggatcg gaccgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
      241 tccccggagt taatccggga cctttaattc aacccaacac aatatattat agttaaataa
      301 gaattattat caaatcattt gtatattata aaatactata ctgtaaatta cattttattt
      361 acaatcaaag gagatatacc atggcacacc atcaccacca tcacgggagc ggggagcaga
      421 aactaatatc agaggaggat cttggctcag gatcctccac cgacggcatg gacgagctgt
      481 acaagggtgc tagcggagga atggtgagca agggcgagga gaccacaatg ggcgtaatca
      541 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      601 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      661 aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc gcctacggca
      721 acagggcctt caccaagtac cccgacga
//
