LOCUS       sequence-136252-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            Adenovirus production).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pAD/CMV/V5-DEST-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..49)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(212..235)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg
       61 ggccctctag atcaaccact ttgtacaaga aagctgggtc tagatatctc gagtgcggcc
      121 gctcgatctc agtggtattt gtgagccagg gcattggcca caccagccac caccttctga
      181 taggcagcct gcacctgagg ttaatcactt agtgatggtg atggtgatgg tggtgctcga
      241 gtcatagcag cacacacctg cggctcttct tcggttctgg aggctccagg gcagccaata
      301 ttgcttcatc aaacacattc tttaggcctt tctgtgtgag ggcagagcac tccacatact
      361 tgacagcctt cagatcccgc gccagctttt cagcagtctc tggagtaata ggcttctgtt
      421 tgttcttggc aagtttctca atagtagagg ggtcatctct gagatcaatt tgggtcccaa
      481 caagcaagaa aggagtcttt ggacagtggt gagttatctc aggcacccac ttttctttca
      541 cattttcaaa tgaggatgga gagaccactg agaaacatac tagaaaaaca tctgtctgtg
      601 gataacttag cggtcgtagt ctgtcataat cctcttgccc tgcagtatca aaaagtccaa
      661 gagtgtatgg ctctccacca atcataactg tgactgcata gttgtcaaaa acag
//
