LOCUS       sequence-136260-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and Venus 
            reporter from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen 
            DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, 
            Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE
  TITLE     BCL11A enhancer dissection by Cas9-mediated in situ saturating 
            mutagenesis.
  JOURNAL   Nature. 2015 Sep 16. doi: 10.1038/nature15521.
  PUBMED    26375006
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 16. doi: 10.1038/nature15521."
COMMENT     Sequence Label: lentiCas9-Venus
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..69)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(70..117)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atctccggct tgtttcagca gagagaagtt tgttgcgccg gatcccttat cgtcatcgtc
       61 tttgtaatct ttcttcttct tagcctgtcc agccttcttt gtggcggcag gtcgcttgtc
      121 gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat
      181 cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc
      241 aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt
      301 ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc
      361 cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc
      421 gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga
      481 gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag
      541 ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt
      601 ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt
      661 gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc tttccatgat
      721 ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct tttccacttt
      781 ggccaccacc agcacagaat aggccacggt ggggctgtcg aagccgccgt acttcttagg
      841 gtcccagtcc ttctttctgg cgatcagctt atcgctgttc ctcttgggca ggatagactc
      901 tttgctgaag ccgcctgtct gcacctcggt ctttttcacg atattcactt ggggcatgct
      961 cagcactttc cgcacggtgg caaaatcccg gcccttatcc cacacgatct ccccggtttc
     1021 gccgtttgtc tcgatcagag gccgcttccg gatctcgccg ttggccaggg taatctcggt
     1081 cttgaaaa
//
