LOCUS       sequence-136261-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a renilla luciferase-YFP fusion protein under control of 
            the rat insulin 2 gene promoter (RIP7) suitable for transfection or 
            generation of transgenic animals.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ghislain J, Fontes G, Tremblay C, Kebede MA, Poitout V
  TITLE     Dual reporter-beta-cell specific male transgenic rats for the 
            analysis of beta-cell functional mass and enrichment by flow 
            cytometry.
  JOURNAL   Endocrinology. 2015 Dec 15:en20151550.
  PUBMED    26671180
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2015 Dec 15:en20151550."
COMMENT     Sequence Label: RIP7-RLuc-YFP-pBS
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     71..87
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(618..869)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(660..681)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1009..1028
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaccatgaa ttacgccagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc
       61 gggccccccc tcgaggtcga cggtatcgcg ataagcttaa aaccctagtg aggctggatt
      121 tctgattaat cccagacttt gtaaagggta ctcagtattc agacataggg gaagtacaca
      181 tactcctcag ggccctcctc tccaccaaga actggctcag gcacaaaaac cacgtcacaa
      241 cccaaggtgt ttattatttg taaaaaaaaa agaacacagt ttggagaaga tgactccatt
      301 ctcagaaagt tccacagcgg aggaagaact ttgtgatatt tgtcacagag gaattattat
      361 aaatggtgga agatagaata tagatacagg tgggagatgg agaaccccag ccagacatta
      421 gatcagagaa cctgctgcca ttcttgaggc ttaatgctgg aaactgaaga agatgaggtc
      481 tccgctgaga tgaacaactg aaaggggtag cctcagacgc cagatccatc gataccggtg
      541 tcctgcaggg ttatctagat ccggtggatc ccgggcccgc ggtaccacgc ctcaacgttt
      601 agttccgatc gccctgcctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      661 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      721 tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      781 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      841 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
      901 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      961 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     1021 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgg
//
