LOCUS       sequence-136269-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in Mammalian Cells, Cas9 Nuclease to analize 
            translocations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, 
            Sfeir A
  TITLE     Mammalian polymerase theta promotes alternative NHEJ and suppresses 
            recombination.
  JOURNAL   Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 
            2015 Feb 2.
  PUBMED    25642960
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14157"; journalName: "Nature"; date: "2015-02-12- 
            12"; volume: "518"; issue: "7538"; pages: "254-7"
COMMENT     Sequence Label: pX330(puro) Rosa26-H3F3B
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(318..338)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(345..410)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggaagaagc tgtcgtccac cttggccatc tcgttgctga agatctcttg cagatagcag
       61 atccggttct tccgtctggt gtatcttctt ctggcggttc tcttcagccg ggtggcctcg
      121 gctgtttcgc cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat gctgtgccgg
      181 tcggtgttgc ccagcacctt gaatttcttg ctgggcacct tgtactcgtc ggtgatcacg
      241 gcccagccca cagagttggt gccgatgtcc aggccgatgc tgtacttctt gtcggctgct
      301 gggactccgt ggataccgac cttccgcttc ttctttgggg ccatcttatc gtcatcgtct
      361 ttgtaatcaa tatcatgatc cttgtagtct ccgtcgtggt ccttatagtc catggtggca
      421 ccggtccaac ctgaaaaaaa gtgatttcag gcaggtgctc caggtaatta aacattaata
      481 ccccaccaac caaccatccc ttaaaccctt acctcttgct cagctaatta cagcccggag
      541 gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg
      601 ggcggcgcga g
//
