LOCUS       sequence-136270-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express in mammalian cells  human PolQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, 
            Sfeir A
  TITLE     Mammalian polymerase theta promotes alternative NHEJ and suppresses 
            recombination.
  JOURNAL   Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 
            2015 Feb 2.
  PUBMED    25642960
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14157"; journalName: "Nature"; date: "2015-02-12- 
            12"; volume: "518"; issue: "7538"; pages: "254-7"
COMMENT     Sequence Label: pcDNA 3.1(Hygro) myc-hPolQ-Flag
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..159
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatctgc
      121 ggccgcatgg aacaaaagtt gatttctgaa gaagatttgt taagaaagag agggatcctg
      181 aatcttctgc gtcggagtgg gaaacggcgg cgttcagaat caggctcaga ttcgttctcg
      241 ggaagcggcg gtgacagcag tgccagcccc cagttcctct ccgggtccgt gctgagcccg
      301 ccgcccggcc ttggtcgctg cctgaaggcc gcagctgcag gagaatgcaa gcctacagtt
      361 cctgactacg aaatagacaa gctactattg gcaaactggg gacttcctaa agcagttctg
      421 gaaaaatacc acagttttgg tgtaaaaaag atgtttgaat ggcaggcaga gtgccttttg
      481 cttggacaag tcctggaagg aaagaattta gtttattcag ctcctacaag tgctgggaag
      541 actcttgtgg cagaattact tattttgaag cgggttttgg aaatgcggaa gaaagctttg
      601 tttattcttc cctttgtttc tgtggctaaa gagaagaaat actacctcca gagtctgttt
      661 caggaagtag gaataaaagt agacggttat atgggcagca cctctccatc aaggcatttc
      721 tcttcattgg atattgcagt ctgcacaatt gagagagcca atggtctgat caatcgcctc
      781 atagaggaaa ataagatgga tctgttagga atggtggttg tggatgaatt acatatgctg
      841 ggagactctc accgagggta tctgctggaa cttttgctga ccaagatttg ctatattact
      901 cggaaatcag catcttgtca ggcagatcta gccagttctc tgtctaatgc tgtgcaaatc
      961 gttggcatga gtgctaccct tc
//
