LOCUS       sequence-136273-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ColK-based vector; provides a nicking function to permit conjugal 
            transfer and bears a polylinker for insertion of methylase-encoding 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
  TITLE     Reduction of conjugal transfer efficiency by three restriction 
            activities of Anabaena sp. strain PCC 7120.
  JOURNAL   J Bacteriol. 1997 Mar;179(6):1998-2005.
  PUBMED    9068647
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1997-03"; volume: "179(6)"; pages: "1998-2005"
COMMENT     Sequence Label: pRL542
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
       61 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      181 gagcgtcaga ccccgtacat cacgaatata gtttgcttga catcctgaca agaataatat
      241 tgtaattaga aaaattatta ttttcatatt tctttccaac aaaagtaaaa atacttatgc
      301 atttaaaata ctacctacat aatttacctg aatcacttat accatggatt cttattttaa
      361 tatttaacga caatgataac actcctttgt tatttatatt tatatcatca atacatgtat
      421 tgctatatcc atactctaaa ttaaccatat ctagatatat caaagaaaat acaaagttaa
      481 aaaaagaacc ctggtactta tgcaagttat ctgcattgtt ttatttatta atggcaatcc
      541 cagtaggatt gccaagtttc atatattaca ctctaaagag aaattaaatc cctaacaact
      601 cattaagttt gctcaactca tcatccccta tgtaagaaga aacaatacct gtgacaattg
      661 caatccccca gaaaccaaga ggcacaccaa caataaaact aaacattaat gccacaattt
      721 ttgccacacc tacatcgacc gcacttttct ccagcgtgac aaacaaaggt cgccagttac
      781 cagtttctat tgcattttta aaatcagaac caacatcata tagaaaagat actcgactgg
      841 tgattttaag cgactttgat atcttcgtta aattcttaga taactcatca taattaacag
      901 actctaacgc attaa
//
