LOCUS       sequence-136274-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ColK-based vector; provides a nicking function to permit conjugal 
            transfer and bears a polylinker for insertion of methylase-encoding 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
  TITLE     Reduction of conjugal transfer efficiency by three restriction 
            activities of Anabaena sp. strain PCC 7120.
  JOURNAL   J Bacteriol. 1997 Mar;179(6):1998-2005.
  PUBMED    9068647
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1997-03"; volume: "179(6)"; pages: "1998-2005"
COMMENT     Sequence Label: pRL542
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttccggtgat gttcaggtta aatccgtcaa aactcaccca cggtaatgat gatatattca
       61 ggtgattctc cggtaaattt ccctgcggaa acaatctggt atcatgctga tattctgccg
      121 ttaccgcatt ataacctgcc ataaactcac tgagatccgg aaaataacgg caggacagtg
      181 cagagaatgt ttcggtttct ttatgaaaga cagtaaagac cgggtctgac tggtcccagt
      241 aaataagttc attgtctttc agtgccatcc ggaactccgg aaactgatta acagcccggg
      301 agatcaggta aatcatcagc ggataaaact tataacctgt ctccgccagt gcggtacgca
      361 aagcggtaat atcgagtttg gtggtcaggc tgaatccgca tttaatctgc tgacgataaa
      421 gggcaaagtg ttccctgcga ttccaggtat tcaggtcaat ccgggtaaaa ttcatggtta
      481 ttccttctga ttaatagtga aaaatattaa taatcagaag gcagtctggt tgtctcaatg
      541 ggtaacattc cgtcctccgt aagctgtttg gtattcagta ataataccct atacgggctt
      601 aatctgtatt aagcccggct ttatttattc cggccaatca tccgcaaaca catagcggat
      661 cagttctgcg gattcacggg gcggtgctct cagcacatcc gccattaaat caatctccat
      721 ctgacaggtt tgcagcttgt cttccgccgg tacatacgga tcatccgtca ggaaactatc
      781 gccgtattta tccatcgacc cctgtatttg tgccgaaaat aaagtcacgg tactctccgg
      841 aggccttttt catatccggc gggcctgaca cttccggatg cagcacacga aaacagaagt
      901 caccggaaca cgccattctg agaa
//
