LOCUS       sequence-136279-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a toehold mutant of the circRAJ31 riboregulator encased in
            a permuted intron exon ribozyme, which circularises the 
            riboregulator. This mutant does not activate cisRAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: circRAJ31-Toehold_mutant
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    97..115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agtctgaggc tcgtcctgaa tgatatgcga ccgccggagg gttgcgtttg agacgggcga
       61 cagatcgaca ctgctcgatc cgctcgcacc gaattctccc tatcagtgat agagattgac
      121 atccctatca gtgatagaga tactgagcac tctagaggtt ctacataaat gcctaacgac
      181 tatccctttg gggagtaggg tcaagtgact cgaaacgata gacaacttgc tttaacaagt
      241 tggagatata gtctgctctg catggtgaca tgcagctgga tataattccg gggtaagatt
      301 aacgacctta tctgaacata atgctaccgt ttaatattcc taggggatca atattaagct
      361 ttaccgacac cccatccgca attaagtccc tatccgctgt cactgtattg gtctgctagc
      421 atgttttctt gggttaattg aggcctgagt ataaggtgac ttatacttgt aatctatcta
      481 aacggggaac ctctctagta gacaatcccg tgctaaattg taggactcaa ttgatcctta
      541 gcgaaagcta aggatttttt ttctgcaggg atcgacgaga gcagcgcgac tggatcagtt
      601 ctggacgagc gagctgtcgt ccgacccgtg atcttacggc attatacgta tgatcggtcc
      661 acgatcagct agattatcta gtcagcttga tgtcatagct gtttcctgag gctcaatact
      721 gaccatttaa
//
