LOCUS       sequence-136280-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taRAJ31min, a linear riboregulator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: taRAJ31min
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    123..141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(380..396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(380..396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      444..475
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        476..578
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gagtctgagg ctcgtcctga atgatatgcg accgccggag ggttgcgttt gagacgggcg
       61 acagatcgac actgctcgat ccgctcgcac cgaattctcc ctatcagtga tagagattga
      121 catccctatc agtgatagag atactgagca cggatcaata ttaagcttta ccgtcgtctc
      181 atccgcaatt aagtccctat ccgctgtcac tgtattggtc tatccttagc gaaagctaag
      241 gatttttttt ctgcagggat cgacgagagc agcgcgactg gatcagttct ggacgagcga
      301 gctgtcgtcc gacccgtgat cttacggcat tatacgtatg atcggtccac gatcagctag
      361 attatctagt cagcttgatg tcatagctgt ttcctgaggc tcaatactga ccatttaaat
      421 catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc
      481 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      541 ttgagttatc gagattttca ggagctaagg aagctaaaat gagtattcaa catttccgtg
      601 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      661 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      721 atctcaacag cggtaagatc cttgagagtt tacgcc
//
