LOCUS       sequence-136286-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a guide RNA (gRNA) to target human IRF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: gRNA-hIRF-1 #12/pSIR
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgaca tgattacgaa ttaattccaa gctaagtgaa
      241 agactctgtc tcaaaaacca acctgacacc tgagccggag cgttgcctct cactaccata
      301 aatatatata cacaacacac acacacacac acacacacac acacacacac acacacacac
      361 aatcatacac ataaacacaa tcacacacac acacatgcac aatcacacac acacaatcac
      421 acacaaacat acatacacac aatcacacac aaccatacac acatatacac aatcacacac
      481 acaaacacaa tcacacacac acacatcaat caatcaatca atcaatatac caaaaatgat
      541 agctcatacc tgtaatctta gatgtgggaa ggctaaaaca gcaggctacc catgacttca
      601 aggtcaccct gggttacaga gtagctaata gtctagcttg aactatagtg taggatcttt
      661 aaaaaaaaat taaccaacca accaacaaac aaacaaacaa acaacaacaa caacaaatgg
      721 aagacagagg acaaaaaaaa ataaaagaaa aagtagaaag ccaatggaga tactatctta
      781 aaattagaaa tatttatgac acattctgaa tactccatgt ttatttcaag gaagccaatg
      841 taactttaag tactatggcc atttaatgta aatacttaag aaaaaa
//
