LOCUS       sequence-136308-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gq-coupled hM3D DREADD fused with mCherry under the 
            control of human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
            Maratos-Flier E, Roth BL, Lowell BB
  TITLE     Rapid, reversible activation of AgRP neurons drives feeding behavior
            in mice.
  JOURNAL   J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
  PUBMED    21364278
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2011-04"; volume: "121"; issue: "4"; pages: 
            "1424-8"
COMMENT     Sequence Label: pAAV-hSyn-DIO-hM3D(Gq)-mCherry
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    253..286
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    337..370
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtaaggcgcg ccataacttc gtataatgta tgctatacga agttattaag aggtttcata
      301 ttgctaatag cagctacaat ccagctacca ttctgcataa cttcgtataa agtatcctat
      361 acgaagttat tccggagtcg actctagagg atccggtacc ttctcgactg cgctctcagg
      421 cacgacacga ctcctccgct gcccaccgca gactgaggca gcgctgagtc gccggcgccg
      481 cagcgcagat ggtcgcgccc gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct
      541 gggccggcgg cggcgcggac gcgaccaagg tggccgggaa ggggagtttg cgggggaccg
      601 gcgagtgacg tcagcgcgcc ttca
//
