LOCUS       sequence-136316-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
            control of human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
            Maratos-Flier E, Roth BL, Lowell BB
  TITLE     Rapid, reversible activation of AgRP neurons drives feeding behavior
            in mice.
  JOURNAL   J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
  PUBMED    21364278
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2011-04"; volume: "121"; issue: "4"; pages: 
            "1424-8"
COMMENT     Sequence Label: pAAV-hSyn-DIO-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    255..288
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    339..372
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
       61 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      241 aagtaaggcg cgccataact tcgtataatg tatgctatac gaagttatta agaggtttca
      301 tattgctaat agcagctaca atccagctac cattctgcat aacttcgtat aaagtatcct
      361 atacgaagtt attccggagt cgactctaga ggatccggta ccttctcgac tgcgctctca
      421 ggcacgacac gactcctccg ctgcccaccg cagactgagg cagcgctgag tcgccggcgc
      481 cgcagcgcag atggtcgcgc ccgtgccccc ctatctcgcg cctcgcgtgg tgcggtccgg
      541 ctgggccggc ggcggcgcgg acgcgaccaa ggtggccggg aaggggagtt tgcgggggac
      601 cggcgagtga cgtcagcgcg ccttcagtgc tgaggcggcg gtggcgcgcg ccgccaggcg
      661 ggggcgaagg cactgtccgc ggtgctgaag ctggcagtgc gcacgcgcct cgccgcatcc
      721 tgtttcccct ccccctctct gataggggat gcgcaatttg gggaatgggg gttgggtgc
//
