LOCUS       sequence-136324-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide synthetase (PurK) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK130
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             103..111
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgacctcctt acattctccg
      121 cttaagccca atgccgttat tggcattgtg gggggtggcc agcttggccg catgtctgct
      181 gttgctgccg cgcgccttgg ttttcgcacg catattctta cgccagaggc caacggcccg
      241 gcggctcagg tctctcacgc cgtaacatgc ggcaagtatg atgatccgac agcactggat
      301 gcctttgccc gcgctgttga tgttgttacc tttgaatttg aaaacatcag cgcagatgca
      361 ctggaccgcc tagccaaaca ctgccctgtt catccttcgg ggcatattct gcgcatcagt
      421 caggaccgat tggcagaaaa aacttttttt gcttctgcgg gtattccact cgcgccatgg
      481 catcctgtta ccgatgtcaa aacgctacac gaagccgcag aaaaagtggg tttgccgctg
      541 attctcaaaa ccactcgcaa tggatatgat ggcaaaggcc agcgccgtgt gcatcacatc
      601 catgatctgg aagaggcttt caacgctctg gcaccacatc ctctggttgc agaaggtatg
      661 gtggattttg cttgcgagat tagtgttatg gtggcgcgtg gtgcagatgg cacggtaagg
      721 tgctttgacc cggcgctgaa ccggcactgg aacggtattc tggatctaac attagccccc
      781 gcccccattg acccagatat tgccacgcag gccataaaac tagccaccac cattgcagaa
      841 aagctggagc tggttggtat tctgggcgtt gaaatgtttg ttgattgcac cggcaacctg
      901 ctggtgaatg aaatggcccc gcgcccccat aattctggcc attggaccat gaatgcctgc
      961 ccgcaggacc aatttgacat gcatatccgt gcagttgcgg ggctgccttt gccgcacgct
     1021 gtgcgccatt ctgatgcaat catgaaaaat ctgttcgggc cggaagatat ggcgctgctg
     1081 ccgcagatta tggccacgca gggctttatt ccgcatctat
//
