LOCUS       sequence-136325-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc and copGFP dual reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..587
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             603..659
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agattctaga
       61 gctagggatc caccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag
      121 ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc
      181 tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga
      241 tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg
      301 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
      361 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
      421 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
      481 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
      541 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgaag cttggaagcg
      601 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta
      661 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
      721 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca
      781 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagc
//
