LOCUS       sequence-136341-       13853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for human erythrocyte surface proteins encoding 
            CR1-isoformB with rat CD4d3+4-bio.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 13853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: CR1-isoformB-CD4d3+4-bio
FEATURES             Location/Qualifiers
     source          1..13853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    659..714
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(713..732)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2950..2968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3036..3140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3141..3209,3210..4001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3359..3378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4172..4760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4661..4680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4914..4931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5048..5069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5084..5089,5090..5107,5108..5114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5122..5138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5127..5149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5146..5162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5146..5162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5433..5812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5813..6016
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5967..5987
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6013..6037
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6518..6537
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6571..6580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggat cactgcatca tattctggat gcacagaaaa tggtgtggaa
      601 tcatcgttaa gtctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa
      661 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
      721 aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga
      781 tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg
      841 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca
      901 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata
      961 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc
     1021 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa
     1081 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc
     1141 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt
     1201 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat
     1261 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta
     1321 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg
     1381 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca
     1441 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc
     1501 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg
     1561 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt
     1621 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa
     1681 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg
     1741 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa
     1801 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg
     1861 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag
     1921 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg
     1981 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg
     2041 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc
     2101 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca
     2161 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga
     2221 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc
     2281 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac
     2341 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta
     2401 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     2461 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     2521 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta
     2581 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa
     2641 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct
     2701 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     2761 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     2821 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     2881 gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa
     2941 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3001 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3361 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     3421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3661 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     3721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5041 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5101 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5161 accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc
     5221 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa
     5281 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     5341 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     5401 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     5461 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     5521 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     5581 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     5641 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     5701 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     5761 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     5821 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     5881 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5941 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     6001 ctatataagc agagctcgtt tagtgaaccg tcagatcctc actctcttcc gcatcgctgt
     6061 ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta
     6121 ctcttggatc ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg
     6181 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc
     6241 aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg
     6301 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga
     6361 ggtgtggcag gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt
     6421 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc
     6481 gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc
     6541 cactcccagg tccaagttta aactgcggcc gccaccatgg gcgccagcag ccctagaagc
     6601 cctgagcctg tgggacctcc tgcccctggc ctgcctttct gttgcggagg cagcctgctg
     6661 gctgtggtgg tgctgctggc cctgcctgtg gcctggggcc agtgcaatgc ccccgagtgg
     6721 ctgcctttcg ccagacccac caacctgacc gacgagttcg agttccccat cggcacctac
     6781 ctgaactacg agtgcagacc cggctacagc ggaaggccct tcagcatcat ctgcctgaag
     6841 aacagcgtgt ggaccggcgc caaggaccgg tgcagacgga agtcctgcag aaaccccccc
     6901 gaccccgtga atggcatggt gcacgtgatc aagggcatcc agttcggcag ccagatcaag
     6961 tacagctgca ccaagggcta ccggctgatc ggcagcagca gcgccacctg tatcatcagc
     7021 ggcgacaccg tgatctggga caacgagaca cccatctgcg accggatccc ttgtggcctg
     7081 ccccccacca tcaccaacgg cgacttcatc agcaccaacc gggagaactt ccactacggc
     7141 agcgtggtca cctacagatg caaccccggc agcggcggca gaaaggtgtt cgaactcgtg
     7201 ggcgagccca gcatctactg caccagcaac gacgaccagg tcggcatttg gtctggccca
     7261 gccccccagt gcatcatccc caacaagtgc acccccccca acgtggagaa cggcatcctg
     7321 gtgtccgaca accggtccct gttcagcctg aacgaggtgg tggagttcag atgccagccc
     7381 ggcttcgtga tgaagggccc cagacgcgtg aagtgccagg ccctgaacaa gtgggagccc
     7441 gagctgccta gctgcagcag agtgtgccag ccccctcctg atgtgctgca cgccgagcgg
     7501 acccagcggg acaaggacaa cttcagccct ggccaggaag tgttctacag ctgcgagccc
     7561 ggctatgatc tgagaggcgc cgctagcatg agatgcaccc cccagggcga ttggagccct
     7621 gccgccccta cctgtgaagt gaagtcctgc gacgacttca tgggccagct gctgaacggc
     7681 agagtgctgt tccccgtgaa tctgcagctg ggcgccaagg tggacttcgt gtgcgacgag
     7741 ggcttccagc tgaagggcag ctccgccagc tattgcgtgc tggccggcat ggaaagcctg
     7801 tggaacagca gcgtgcccgt gtgcgagcag atcttctgcc ccagccctcc cgtgatcccc
     7861 aatggcagac acaccggcaa gcccctggaa gtgttcccct tcggcaagac cgtgaactac
     7921 acctgtgacc cccaccccga cagaggcacc agcttcgacc tgatcggcga gagcaccatc
     7981 agatgcacca gcgaccccca gggcaatggc gtgtggagca gccctgcccc tagatgtggc
     8041 atcctgggcc actgccaggc ccccgaccac ttcctgttcg ccaagctgaa aacccagacc
     8101 aacgccagcg actttcctat cggcacaagc ctgaagtatg agtgccggcc tgagtactac
     8161 ggcaggccct tctccatcac ctgtctggac aacctcgtgt ggtccagccc caaggacgtg
     8221 tgcaagagaa agagctgcaa gaccccccct gatcctgtga acgggatggt ccatgtgatc
     8281 accgacatcc aggtcggctc ccggatcaac tactcctgca ccaccggcca cagactgatc
     8341 ggccacagct ccgccgagtg catcctgtct ggcaacgccg cccactggtc caccaagccc
     8401 cccatctgcc agagaatccc atgcggcctg cctccaacaa tcgccaatgg ggatttcatc
     8461 tccaccaacc gcgaaaattt tcactatggc tccgtcgtga cctaccggtg caatcccggc
     8521 tctggcggac ggaaagtctt tgaactcgtc ggcgaacctt ccatctactg tacctccaat
     8581 gatgatcagg tcgggatttg gagcggaccc gcccctcagt gtatcattcc taataagtgt
     8641 acccctccta atgtggaaaa tgggatcctc gtgtccgata acagaagcct gttctccctg
     8701 aatgaagtgg tcgagttccg gtgtcagcct ggatttgtga tgaaaggccc tcggcgggtc
     8761 aagtgtcagg ctctcaataa gtgggaacct gaactgccct cctgctctag agtgtgtcag
     8821 cctccccccg acgtcctgca tgccgagaga acccagagag ataaggacaa tttctccccc
     8881 ggacaggaag tcttttactc ttgcgagcct ggatacgacc tgaggggcgc tgcctccatg
     8941 agatgtacac ctcaggggga ctggtcccca gccgccccaa catgcgaggt caagagctgt
     9001 gacgacttta tggggcagct cctcaatggg cgggtgctgt ttcctgtcaa cctccagctg
     9061 ggggctaaag tggattttgt gtgtgatgaa ggatttcagc tgaaaggctc cagcgcctcc
     9121 tactgtgtgc tggctgggat ggaatctctg tggaattcct ccgtgcctgt gtgtgaacag
     9181 attttttgcc cctccccccc tgtgatccct aacggccggc acacagggaa gcctctcgag
     9241 gtgttccctt ttggaaagac agtgaattat acatgcgacc ctcaccctga tcggggcacc
     9301 tcttttgatc tgatcgggga gtccaccatc cggtgcacat ccgatcctca ggggaacggc
     9361 gtctggtcct ctcctgcccc acgctgcggc attctggggc attgtcaggc ccctgatcat
     9421 tttctgtttg ccaaactcaa gacccagaca aatgcctccg acttcccaat cgggacatcc
     9481 ctgaagtacg aatgtaggcc cgagtactat ggccgccctt ttagcatcac atgcctggat
     9541 aatctcgtct ggtcatcccc caaagatgtg tgcaaacgca agtcttgtaa gacacctccc
     9601 gacccagtca acggaatggt gcatgtcatt acagatattc aggtcggcag cagaatcaat
     9661 tactcttgta caaccggaca ccggctgatt ggccactcca gcgccgagtg tattctgagc
     9721 ggaaatgccg cccattggag cacaaagcct ccaatctgcc agcggattcc ctgtgggctg
     9781 ccacctacca ttgctaatgg cgactttatc tctaccaaca gagagaattt ccattacgga
     9841 tctgtggtca cataccggtg taaccctggc tccgggggca ggaaagtgtt cgaactcgtc
     9901 ggagagccat ccatctattg cacaagtaat gacgatcagg tcggcatttg gagtggccct
     9961 gctccacagt gtattatccc taacaaatgc acacctccaa atgtcgagaa tggaattctg
    10021 gtgtctgaca atcgcagcct gttttctctc aacgaagtgg tggaatttcg gtgccagcca
    10081 ggttttgtca tgaaggggcc tagaagagtc aaatgccagg cactcaacaa atgggagcct
    10141 gaactcccca gctgtagccg cgtctgccag cctccaccag acgtgctcca cgctgagaga
    10201 acacagaggg acaaagataa cttctctcca gggcaggaag tgttttattc ctgcgaacca
    10261 ggctacgatc tgcgcggagc cgcctctatg aggtgtactc cacagggcga ttggtcaccc
    10321 gccgctccaa cttgtgaagt caaatcctgt gatgatttca tgggacagct gctcaatgga
    10381 cgcgtcctgt ttccagtcaa tctgcagctc ggagcaaaag tcgactttgt ctgtgacgag
    10441 gggtttcagc tcaaggggag cagcgcttct tactgcgtgc tcgcaggaat ggaatccctc
    10501 tggaacagct ctgtgccagt ctgcgaacag atcttttgtc cttctccacc cgtgattccc
    10561 aacgggcggc ataccgggaa accactggaa gtctttccat ttggcaaggc tgtgaattac
    10621 acttgcgatc cacaccctga caggggcaca tccttcgatc tgattggcga atccacaatc
    10681 agatgtacat ccgacccaca gggaaatggt gtctggtcta gccccgctcc cagatgcggg
    10741 atcctcggac actgtcaggc tccagatcac tttctcttcg caaagctcaa gacacagact
    10801 aacgctagcg atttccccat tggcacatct ctcaagtacg agtgtcgccc agagtattac
    10861 ggacggcctt tcagcattac ttgtctcgat aatctcgtgt ggagtagccc aaaggatgtg
    10921 tgtaaaagaa agtcctgtaa aacccctcca gatcctgtca atggaatggt ccacgtgatc
    10981 actgatattc aggtcgggag ccgcatcaac tacagctgta caacaggcca taggctgatc
    11041 gggcactcct ctgctgaatg catcctgagc gggaacaccg ctcactggtc tacaaagcca
    11101 cctatttgtc agaggattcc ttgcggactg cccccaacta ttgctaacgg cgatttcatt
    11161 tccacaaatc gggaaaactt ccattatggg agcgtcgtga catatagatg caacctgggc
    11221 agccggggac gcaaagtgtt tgaactcgtg ggggagccta gtatctattg tacctctaac
    11281 gatgaccagg tcggcatttg gagcgggcct gcaccccagt gcattattcc aaacaaatgt
    11341 actccaccaa acgtggaaaa cggaattctc gtcagcgaca acagatctct gttttcactg
    11401 aatgaggtcg tggagtttcg ctgtcagcct ggcttcgtca tgaaaggacc ccgcagagtg
    11461 aagtgtcagg ctctgaacaa atgggaacca gagctgccct cttgcagtcg cgtgtgccag
    11521 ccacccccag agatcctgca cggcgagcac acccccagcc accaggataa cttttcaccc
    11581 ggacaggaag tgttctatag ttgcgaacca ggatatgacc tcaggggagc cgccagcctg
    11641 cactgtacac cacaggggga ttggagcccc gaagccccca gatgcgccgt gaaaagctgc
    11701 gacgattttc tcggacagct gccccacgga agggtcctgt tccccctgaa cctccagctc
    11761 ggagctaagg tgtccttcgt gtgtgatgag gggtttagac tgaagggatc cagcgtgtcc
    11821 cattgcgtgc tcgtgggcat gcggtccctg tggaataatt ccgtcccagt gtgcgagcac
    11881 attttttgtc ccaaccctcc agccattctg aatggacggc acaccggcac ccctagcggc
    11941 gacatccctt acggcaaaga gatcagctat acttgcgacc cccatcctga ccgcggcatg
    12001 accttcaacc tgattggaga gtctaccatc aggtgtacct ctgaccctca cggaaacggc
    12061 gtgtggtcta gtccagctcc ccgctgcgaa ctgtccgtga gagccggcca ctgcaaaacc
    12121 cccgagcagt tcccctttgc cagccctacc atccccatca acgacttcga gtttcccgtg
    12181 gggaccagcc tgaactatga atgtcgccct ggctactttg gcaagatgtt cagcattagc
    12241 tgcctcgaga acctcgtctg gtctagtgtg gaggacaact gccggcggaa gtcttgtggc
    12301 ccccctcccg agccctttaa cggcatggtc cacatcaaca ccgacaccca gtttggcagc
    12361 accgtgaatt acagctgcaa tgagggcttc agactgattg gcagccccag caccacatgt
    12421 ctggtgtccg gcaataacgt gacctgggat aagaaggccc ctatctgcga gatcatctcc
    12481 tgcgagcccc ctcccacaat ctctaacggg gacttctaca gcaacaacag aaccagcttc
    12541 cacaacggca ccgtcgtgac ttatcagtgc cacaccggcc ctgatggcga gcagctcttt
    12601 gaactcgtgg gagagagaag catctattgc acctcaaagg acgatcaggt cggcgtgtgg
    12661 tccagccctc cccccagatg catctctaca aataagtgca ccgcccctga ggtggagaat
    12721 gccatccggg tgcccggcaa cagaagcttc ttcaccctga ccgagatcat ccggtttagg
    12781 tgtcagccag gtttcgtgat ggtcggcagc cacaccgtcc agtgccagac aaatggcaga
    12841 tggggcccca agctgccaca ctgcagccga gtctgtcagc cacctcccga aattctgcat
    12901 ggggaacaca ccctgtccca ccaggacaat ttcagtccag gccaggaagt cttttacagt
    12961 tgcgaaccct cctacgacct gagaggggcc gcttctctgc actgcactcc ccagggcgac
    13021 tggtcccctg aggcccctag atgcacagtg aagtcttgtg acgatttcct cggacagctc
    13081 cctcatggca gggtgctgct gccactgaat ctccagctgg gtgcaaaagt ctcttttgtc
    13141 tgcgacgaag gctttaggct gaaaggaaga tctgcctccc actgcgtcct ggccggaatg
    13201 aaggctctct ggaactctag cgtccccgtc tgtgaacaga ttttctgtcc caatcctccc
    13261 gctattctga acggccgcca cacaggaacc cccttcggcg atatccccta tgggaaagaa
    13321 atctcctacg cctgcgacac ccacccagat aggggcatga cattcaatct catcggcgaa
    13381 agcagcatcc gctgcacaag tgatcctcag ggcaatggcg tctggtcatc cccagcccct
    13441 cgatgcgagc tgtctgtgcc tgccgcctgc ccccaccccc ctaagatcca gaacggccac
    13501 tacatcggcg gccacgtgtc cctgtatctg cccggcatga ccatcagcta catctgcgat
    13561 cctggctatc tgctcgtggg gaagggcttc atcttctgca ccgaccaggg catctggtcc
    13621 cagctggatc actactgcaa agaagtgaac tgcagcttcc ccctgttcat gaacggcatc
    13681 agcaaagaac tggaaatgaa gaaggtgtac cactacggag actacgtgac cctgaagtgc
    13741 gaggacggct acaccctgga aggcagccct tggagccagt gtcaggccga cgacagatgg
    13801 gacccccctc tggccaagtg caccagcaga acccacgacg ccctgggcgc gcc
//
