LOCUS       sequence-136343-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA against CRTC3 (3rd generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Jeanneteau FD, Lambert WM, Ismaili N, Bath KG, Lee FS, Garabedian 
            MJ, Chao MV
  TITLE     BDNF and glucocorticoids regulate corticotrophin-releasing hormone 
            (CRH) homeostasis in the hypothalamus.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1305-10. doi: 
            10.1073/pnas.1114122109. Epub 2012 Jan 9.
  PUBMED    22232675
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1114122109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-24- 24"; volume: "109"; issue: "4"; pages: "1305-10"
COMMENT     Sequence Label: pLL3.7 shRNA against CRTC3
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(67..270)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(96..116)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    690..723
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(765..781)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(766..782)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct gacggttcac
       61 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      121 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      181 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      241 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      301 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      361 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      421 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      481 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      541 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      601 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
      661 cgcggccgct taagcttgga acccttaata taacttcgta taatgtatgc tatacgaagt
      721 tattaggtcc ctcgacctgc tggaatctcg tgaagcgagc ttatcgatac cgtcgacctc
      781 gagaattcaa aaagaccaat tctgattctg ct
//
